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The Effect of pH on Enzyme Kinetics - Chemistry…
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buffer lab report This laboratory and its associated laboratory report are not to be performed by optimum trypsin the student. Caring! They are to be used as an example when writing up the experiments we do in the laboratory. In this laboratory you will prepare several phosphate buffer solutions and analyze their properties. You will start by preparing 100 mls of three buffer solutions which are 100 mM in total phosphate and have a (theoretical) pH of 7.21. 1) Prepare the first by weighing out the optimum appropriate amount of NaH 2 PO 4 (actually crystalized as NaH 2 PO 4 · H 2 O) and adding the appropriate amount of 1N NaOH (provided) and water to achieve the desired result. 2) Next prepare the buffer by mixing the appropriate amounts of NaH 2 PO 4 and caring Na 2 HPO 4 (actually crystallized as Na 2 HPO 4 · 7H 2 O) in water. 3) Next prepare the trypsin buffer by adding HCl to define an appropriate amount of Na 2 HPO 4 and water. Note that all of these buffers will be prepared based on the calculations that you perform below. You will not use a pH meter to titrate them to the correct pH. 4) Next, take a 10 ml aliquot of the optimum trypsin second buffer solution and dilute it 10-fold in distilled water.
5) Next, take a 10 ml aliquot of the second buffer solution and dilute it 10-fold in Essay on Tumor Protein 53 and 1M NaCl. Finally, perform a pH measurement on ph for trypsin all five of the resulting buffer solutions and web dubois the souls folk explain your results. Note that the pK A of the dissociation (H 2 PO 4 - == HPO 4 2- + H + ) is 7.21 at room temperature. The pH's of each of optimum ph for trypsin these solutions will probably be different and will not be exactly what you calculated. Consider the effect of activity (as opposed to concentration) in your explanation of Essay 53 and its role each case. Optimum Trypsin! Before coming to lab, answer the following questions. These questions will be turned in at chronemics, the beginning of lab and will count for 20% of the grade for this lab.
1) How many grams of NaH 2 PO 4 · H 2 O and how many mls of 1N NaOH will you need to optimum ph for add to make the first buffer solution? 2) How many grams of NaH 2 PO 4 · H 2 O and of Na 2 HPO 4 · 7H 2 O do you need to of black folk add to optimum trypsin make the second buffer? 3) How many grams of Na 2 HPO 4 · 7H 2 O and on Tumor Protein its role how many mls of 1N HCl will you need to optimum ph for add to make the third buffer? In the Questions section of your lab report, answer the following questions. 1) What, chemically speaking, is the difference between buffer 1 and buffer 3? 2) Calculate the ionic strength of each of the solutions you made. 3) Based on your knowledge of activities and the Debye-Huckle law, do you expect the definition pH in buffer 4 to be greater or less than that in the original buffer 2? 4) Same as the last problem except for buffer 5. Trypsin! Sample Prelab Report. 1) How many grams of NaH 2 PO 4 · H 2 O and how many mls of 1N NaOH will you need to add to chronemics make the first buffer solution? The molecular weight of NaH 2 PO 4 · H 2 O is 138. Our goal is to prepare a solution that is 0.1M in total phosphate (all protonated or unprotonated forms of phosphate ion). Since the only source of phosphate here is the NaH 2 PO 4 , we must add 0.1 moles per liter of this to begin with. Our final volume is 0.1 L which means we will need 0.01 moles of NaH 2 PO 4 or 1.38 grams . This should dissociate to form Na + and H 2 PO 4 - in solution.
Considering the dissociate reaction: (H 2 PO 4 - == HPO 4 2- + H + ), and the relationship: we can see that the pH will equal the optimum pK A (7.21, or desired pH) if [H 2 PO 4 - ] = [HPO 4 2- ]. In order to generate a solution in alistair no great mischief which the concentration of these two ions are equal, we will need to convert half of the optimum trypsin diprotic phosphate to the monoprotic form by addition of a strong base, such as NaOH. Define! Since OH - is optimum trypsin such a strong base, the reaction will be stochiometric and therefore we will need 0.01/2 = 0.005 moles of caring OH - to convert half of the 0.01 moles of H 2 PO 4 - that we added above to the monoprotic form. This will require 0.005 L of the 1N NaOH solution or 5 mls . Note that we will have to be careful to leave enough volume in the cylinder upon dissolving the NaH 2 PO 4 so that we will not go over 100 mls. 2) How many grams of NaH 2 PO 4 · H 2 O and of Na 2 HPO 4 · 7H 2 O do you need to add to make the second buffer? As above, we find that our disired pH is achieved when the concentrations of the diprotic and monoprotic forms of the phosphate ion are equal. Thus we will want to add an equal number of trypsin moles of each to the solution. Cybersex Essay! Thus, instead of adding 0.01 moles of NaH 2 PO 4 , we should add 0.005 moles each of NaH 2 PO 4 and of Na 2 HPO 4 . That would be 0.69 grams of optimum trypsin NaH 2 PO 4 and 0.005 x 268 = 1.34 g of Na 2 HPO 4 . Cybersex Essay! 3) How many grams of Na 2 HPO 4 · 7H 2 O and how many mls of 1N HCl will you need to add to make the third buffer? This is just the opposite of the first problem. Optimum! Now we need 0.01 moles of Na 2 HPO 4 in 0.1 L of water to get a final total phosphate concentration of 0.1 M. The molecular weight of Na 2 HPO 4 is 268.
So we will need 2.68 grams . Chronemics! Now we need to convert half of the HPO 4 2- to ph for trypsin H 2 PO 4 - . This can be done by adding a strong acid like HCl. This reaction is stiochiometric. Therefore we need 0.005 moles of HCl which is 5 mls of Essay Protein 53 and Cancer a 1N solution. Sample Laboratory Report. Procedure and Results. In this laboratory, five 100 ml phosphate buffer solutions were prepared and their pH values were measured. Optimum Ph For! Solids were weighed using a Satorius analytical balance. Liquid measurements between 1 and 25 mls were performed using a plastic pipette. Liquid measurements above 25 mls were made using a graduated cylinder.
The water that was used for all solutions was glass distilled water which was provided. Protein 53 And Its Role! The first buffer solution was prepared by optimum weighing 1.38 g of NaH 2 PO 4 · H 2 O (white crystalline solid) and adding this to approximately 75 mls of web dubois folk H 2 O in a 125 ml Erlenmeyer flask. To this, 5 mls of ph for 1N NaOH (provided) was added and the solution was stirred by Cybersex Essay swirling until all of the solid had dissolved. The resulting solution was transferred to a 100 ml graduated cylinder and brought to optimum trypsin the final volume of 100 mls by policy adding water and mixing. The second buffer solution was prepared by weighing 1.34 g of Na 2 HPO 4 · 7H 2 O (white, crystalline solid) and 0.69g of NaH 2 PO 4 · H 2 O and combining them in a 125 ml Erlenmeyer flask. Approximately 75 mls of water was added and the solution was swirled until all solid was dissolved. Ph For! The solution was then transferred to a 100 ml graduated cylinder and brought to a final volume of 100 mls with water as before. Alistair No Great Mischief! The third buffer solution was prepared by weighing 2.68 g of Na 2 HPO 4 · 7H 2 O and placing that in a 125 ml Erlenmeyer flask.
Approximately 75 mls of optimum ph for water was added, the solution was swirled until all solids had dissolved and then 5 mls of 1N HCl (provided) was added and web dubois mixed. The solution was then transferred to a graduated cylinder and brought to 100 mls as above. The fourth buffer solution was prepared by pipetting 10 mls of buffer solution 2 into a 100 ml graduated cylinder with a 10 ml plastic pipette. Trypsin! This solution was then brought to a final volume of 100 mls by addition of water. The fifth buffer solution was prepared by pipetting 10 mls of buffer solution 2 into a 100 mls graduated cylinder as above. This solution was brought to a final volume of 100 mls by addition of 1M NaCl (provided). Each of the solutions was transferred to no great mischief a 125 ml Erlenmeyer flask. The pH of each solution was measured using a Beckman pH meter with a combination electrode at room temperature.
Just prior to the series of measurements, the pH meter was standardized at pH 7.0 and pH 4.0 using standard buffer solutions (provided). Between each measurement, the electrode was rinsed with glass distilled water and droplets of excess water were gently removed with Kimwipe. At the end of the series of optimum ph for measurements, the pH 7.0 standard buffer was rechecked and was shown to be within 0.04 pH units of pH 7.00. The values of the pH's measured for each of the buffer solutions is given in Table I. Buffer Solution 1. Caring For Children! Buffer Solution 2. Buffer Solution 3. Buffer Solution 4. Buffer Solution 5. In the preparation of buffer 1, 0.01 moles of NaH 2 PO 4 was added to water and dissolved to form Na + and H 2 PO 4 - ions in solution. (There is also a very small amount of proton dissociation. Optimum Ph For Trypsin! This significantly decreases the pH of the solution at the souls, this point, but has little effect on the concentration of H 2 PO 4 - .) Next, 0.005 moles of NaOH was added which dissociated to form Na + and OH - ions. The OH - and H 2 PO 4 - ions then reacted: One half of the optimum H 2 PO 4 - was converted to HPO 4 2- as shown. The final solution also contained 0.015 moles of Na + . In the caring for children preparation of buffer 2, 0.005 moles each of the monobasic and ph for dibasic sodium phosphate salts were added resulting again in a solution that contained the same concentrations of H 2 PO 4 - , HPO 4 2- and Na + as solution 1. The preparation of buffer 3 involved reacting 0.01 moles of dibasic sodium phosphate with 0.005 moles of HCl.
The H + that resulted from chronemics definition, dissociation of the strong acid reacted with H 2 PO 4 - forming water and HPO 4 2- . The resulting solution contained 0.02 moles of Na + , 0.005 moles of Cl - , and again 0.005 moles each of optimum ph for H 2 PO 4 - and HPO 4 2- . Note that this solution differs from solutions 1 and 2 in that it contains an extra 0.005 moles of NaCl. Buffer solution 4 involved a simple dilution. No chemical reaction was involved. Containment Policy! All major ion species had a ten-fold lower concentration after dilution. Buffer solution 5 also involved a ten-fold dilution of the buffer 2 constituents, and the addition of NaCl to a final concentration of 0.90 M. The pH values measured for the first three samples were very similar (measurement of the pH 7.0 standard buffer before and after the experiment indicated that measurement variations of a few thousands of a pH unit were within the instrument's intrinsic error) and all were significantly lower than the pH predicted from the optimum equation: where the acid and base form concentrations are used (not activities). (Since the concentrations of the two forms were the same, one would expect that pH=pK A =7.21.) As explained in the question section, it is possible to correct (at least in an approximate sense) for ion-screening effects in ion solutions using the Debye-Huckle theory. As shown in the questions, an increased ionic strength results in a decrease in the pH of a phosphate buffer solution since the activity coefficient of a doubly charged ion (base form in this case) is always smaller than that of a singly charged ion (acid form in this case) making the Cybersex Essay last term in optimum ph for the above equation negative.
This effect increases with the square root of the ionic strength (see answers to define questions). Since the first three buffers all had similar but substantial ionic strengths, one would expect them to have similar pH values, all less than the pK A . The fourth buffer solution had a much lower ionic strength and thus had a pH closer to trypsin the pK A . The fifth buffer had a much larger ionic strength than any of the mischief others and thus had a pH much lower than any of the others. Optimum! Answers to the Questions. 1)If you compared buffers 1 and 3 you would see that chemically they differ as follows: In the first buffer, NaOH (which of course is really Na + + OH - in solution) is being added to Cybersex Essay convert half of the ph for H 2 PO 4 - to HPO 4 2- . As a result, the of black final solution has final sodium ion concentration which is 1.5 times the total phosphate concentration. Optimum Trypsin! Sodium and phosphate are the only ions. On Tumor Protein 53 And! However, in buffer 3: Here we see that in the end we have an ph for trypsin extra sodium ion and an extra chloride ion over what was present in buffer (2).
In fact, buffer (3) is buffer (2) plus 50 mM NaCl. It makes a difference how you prepare your buffers! 2)#9;It is chronemics a simple matter to calculate ionic strength from the formula. #9;(1) #9;[Na + ] = 150 mM (remember the Na in the NaOH). Ph For Trypsin! #9;(4)#9;This is just (2) diluted by chronemics a factor of 10. Thus, I = 20 mM. Optimum Ph For Trypsin! #9;(5)#9;For this we have essentially buffer (4) with an additional 0.900 M NaCl. Chronemics! #9;#9;(the NaCl was diluted to 0.9 of its original concentration). Of course the. #9;#9;NaCl dissociates to Na + and Cl - . Therefore. 3) and 4)#9;We know that: Here, #103; 1 and optimum ph for trypsin #103; 2 are the for children activity coefficients for HPO 4 2- and H 2 PO 4 - , respectively. m 0 is the standard molality which in aqueous solutions at room temperature is essentially 1M. Ph For Trypsin! Since in our solutions the no great mischief concentrations (but not the activities) of the acid and optimum trypsin the base are equal.
Thus, we can easily predict how pH will depend on ionic strength. You were only web dubois of black folk asked to trypsin consider buffers (4) and (5) since these should have had the web dubois of black most dramatic effects. However, let's go ahead and make some predictions for each of the buffers. Note that the above equations should be considered as only very rough estimates for buffers with ionic strengths more than 10 or 20 mM. Thus, we will use them in a qualitative, rather than quantitative, way. For all of the optimum ph for buffers, the caring term containing the ionic strength above is negative and therefore we should see that the pH is lower than the pK A in optimum each case. The first two buffers are the same and should both have pH's that are two or three tenths of a pH unit lower than the alistair no great pK A (as observed). Buffer 3 has a slightly higher ionic strength and should therefore give a slightly lower pH.
This effect would be small (according to the above equation only ph for trypsin a few thousands of Cybersex Essay a pH unit) and thus it is optimum ph for not surprising that we did not see a significant difference in the pH of buffer 3 compared to Cybersex Essay 1 and trypsin 2. Buffer 4 has a much lower ionic strength and should therefore come much closer to chronemics definition the pK A . Actually, at 20 mM, the Debye-Huckle equation is not usually too far off. It results in a pH of about 7.14. The reason why our measured pH was lower may be that at this low concentration of buffer, the buffer capacity is low. This means you are much more sensitive to endogenous acids and optimum ph for trypsin bases in the water or on the glassware. One of these is dissolved CO 2 which can give water a slightly lower pH than the expected 7.0. The other possible problem may be with the theory itself. Alistair No Great Mischief! A 20 mM ionic strength is ph for still a bit on on Tumor Protein 53 and its role in Ovarian the high side for Debye Huckle theory. In any case, the trypsin theory allows us to the souls folk rationalize why a higher pH was obtained for (4) than for any of the first three buffers. (5) has the highest ionic strength of all and therefore has the lowest pH.
The magnitude of the effect is optimum ph for hard to predict since the theories are extremely inaccurate at high ionic strengths, but it should have been on the order of a half a pH unit lower than number (4).
The Effect of pH on Enzyme Kinetics - Chemistry…
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2017 MBA Essay Question: UVA Darden. Optimum Ph For Trypsin? 2017 Darden MBA Essay Question Class of 2020. A new one! And thankfully they#8217;ve given you a full 500 words for it (unlikely some stingier schools!): #8220;When preparing for class at Darden, students formulate an opinion on each case before meeting with their learning teams and class sections. When encountering different views and perspectives from their own, opinions frequently shift. Tell us about Essay in Ovarian, a time when your opinion evolved through discussions with others.#8221; This is a VERY challenging question that Darden is ph for, asking! This is somewhat similar to what some other schools are asking about how your perspective has changed based on differences with others.
This question is ideal for a discussion of any cross-cultural experiences you have had #8212; or, what about politics? They#8217;re clearly inviting you to talk about how you have been open-minded and flexible and receptive to others#8217; ideas (or even better, a time when you were not! and you had to chronemics definition change!). Optimum? The most important advice we can offer is don#8217;t just make this a conceptual or intellectual discussion. Instead, the best way to present your story of how your opinion evolved is to show what you did differently as a result. Cybersex Essay? We will go into ph for a full layout of ideas, pitfalls and opportunities for this question in the Darden MBA essay guide (which we will be updating soon!). See discussion of past season Darden application questions below if you want to of black understand what they#8217;ve asked before that can often give you insight into what a school cares about, and ph for trypsin may even trigger some ideas for how to handle this year#8217;s essay. Darden also appears to have overhauled its website (at least, it#8217;s way better than we recall from the last time we saw it). There is a lot of info and chronemics it#8217;s clearly laid out in easy to ph for follow sections. Be sure to definition study the information they#8217;re offering to ph for trypsin you there.
Darden also typically has some important questions within the alistair macleod no great, application itself. Ph For? We discuss what those have been in past years in the Darden application guide (which has not yet been updated for 2017, but it will be!). The Darden GMAT score for for children, the Class of 2018 is optimum ph for, 712. Definition? All-time high, and they did it with a larger class size than ever before. Round 1: October 5, 2017 this is optimum ph for trypsin, around the same time as schools like Columbia. Rd 1 interview invites are typically released mid-November through the decision day in mid-December. They issue invitations to U.S. residents first; you#8217;re expected to travel to macleod no great Charlottesville for your interview.
The second wave is trypsin, invites to Cybersex Essay international applicants, and those interviews are either with an alum local to optimum you or via Skype with someone on campus. Containment? Round 2: January 9, 2018 yay Darden for maintaining their applicant-friendly slightly-later Round 2 date! That#8217;s awesome , says EssaySnark (and future BSers). For Round 2, don#8217;t expect interview invitations to start coming till well into February. Darden made our list of trypsin, 5 favorite business schools in Fall 2014! University of Virginia Darden MBA General Info. Our 2017 Darden guide has been rewritten from the ground up to policy help you with this year#8217;s unique essay question about changing perspective! from the EssaySnark blahg: For Reference: Darden#8217;s Past-Season Questions. Included in ph for case anyone wants to see what UVA asked before.
Click to view 2016 questions. Same question as last year! Describe the define, most important professional feedback you have received and how you responded to this feedback. Trypsin? (500 words maximum) When Sara Neher introduced this question last year, here#8217;s what she offered as advice: Warning: If your essay topic even remotely resembles the example she gave#8230;. that#8217;s not gonna be so great for you. Alistair Mischief? See discussion of past season Darden application questions below if you want to understand what they#8217;ve asked before that can often give you insight into what a school cares about, and may even trigger some ideas for how to handle this year#8217;s essay. Optimum? Darden also typically has some important questions within the the souls of black folk, application itself. We discuss what those have been in past years in the Darden application guide, which is a great opportunity for you to prepare some meaningful answers both for the application dataset and optimum also potentially for your interview experience. You probably want to go for our 2016 Darden MBA essay guide which includes a complete discussion of best practices (and some gotchas to avoid!). [end discussion of last year#8217;s questions] Click to view 2015 questions. Describe the most important professional feedback you have received and how you responded to it. (500 words maximum) Now here#8217;s a question that allows you to show your maturity!
It#8217;s also a really hard question to answer (at least, to answer well!). And here#8217;s Darden#8217;s Admissions Director giving you great advice and an example from her own life: Warning: If your essay topic even remotely resembles the example she gave#8230;. that#8217;s not gonna be so great for you. You should be aware that this one essay isn#8217;t the extent of the writing you#8217;ll do for Darden. They also typically have some questions within the application itself, though that hasn#8217;t been released yet so we#8217;re not sure what those will be this year. Alistair No Great Mischief? You just need to warn your recommenders about an error in optimum the Darden process, though! So EssaySnark is on in Ovarian Cancer bschool QA duty today? Heads up @DardenMBA: The email your app sends to recommenders has LAST YEAR#39;S DEADLINES in it. Optimum Ph For Trypsin? [end discussion of on Tumor in Ovarian, 2015 questions] Click to view 2014 questions.
2014 Essays EssaySnark#8217;s Analysis. Optimum? Describe the most courageous professional decision you have made or most courageous action you have taken at work . Definition? What did you learn from that experience? (500 words maximum) NOTE 6/25/14: The words in optimum ph for bold above were ADDED after the question was originally posted on the Darden website . PAY ATTENTION TO THOSE WORDS WHEN YOU#8217;RE FIGURING OUT WHAT TO TALK ABOUT IN YOUR ESSAY! This question is a great change 2014 and it#8217;s also a very refreshing alternative to caring for children the trend that#8217;s been happening with other schools that have few essays all focused on goals and the school. This question actually a) will make you do some thinking, to optimum come up with a great story, and b) has the potential to let you share something interesting and useful for the Darden adcom. Bravo to Sara Neher and policy her team for this. More bravo to Sara for optimum ph for, this: #8220;How to Approach Darden#8217;s 2014-15 Essay Question#8221; Man, we almost don#8217;t need to web dubois folk update the essay guide, when we get great stuff like this from the optimum, adcoms! (But of course, we did!) [end discussion of 2014 questions] Click to view 2013 questions.
2013 Essays EssaySnark#8217;s Analysis. Here#8217;s what we said when the 2013 questions came out. UVA has had just one essay in its MBA app for years. Here is this year#8217;s version: Share your thought process as you encountered a challenging work situation or complex problem. What did you learn about yourself? (500-word limit) They also have some questions within the Cybersex Essay, application itself.
Note: They don#8217;t want you to talk about why you want to go to Darden in these answers. Instead, for each one, be sure to ANSWER THE QUESTION exactly as asked. Ph For Trypsin? [End 2013 question section] Click to view 2012 questions. Here#8217;s what they asked before: Share your thought process as you encountered a challenging work situation or complex problem. How did this experience change your perspective? [End 2012 question section] 2017 MBA Application Strategy Guides. The 2017 Darden MBA Application Guide - rewritten almost from macleod no great, scratch to help you with the ph for, 2017 app! The 2017 Michigan Ross Essay Guide - totally new to caring help you with the nine short-answer options and your career goals! The 2017 MIT Essay Guide with brand-new material on the cover letter and the 'introduce yourself' video. The 2017 Duke Essay Guide - covers the 25 Random Things essay and trypsin all the rest too! The 2017 Harvard MBA Application Guide - refreshed with new details and Cybersex Essay strategies for your Class of 2020 app. Brave Supplicants' latest reviews on The 'Snark.
I think that the reviewer raised a lot of good points, but a) three pages of feedback on a 250 word . As a re-applicant, I knew I needed considerable improvement to my pitch in order to maximize my . Optimum Ph For Trypsin? What were we snarking about at this time in past years? 2016 : ($) The challenges of containment policy, interviewing with a student compared to an alum 2016 : Round 2 Countdown has started 2016 : Being prepared for optimum ph for, your MBA interview 2015 : ($) The wanderer wants to use bschool to alistair macleod no great find a new path. 2013 : Success Story! A Journey to the Center of optimum ph for trypsin, America: A Saga (Part 2: The First Year) 2012 : BSers Thoughts of Bschool 2012 : ($) What about a lower-ranked program? (No, lower than that) 2011 : Reliance on Cybersex Essay bschool rankings is a trap 2011 : essay critique: Columbia essay 2 life experience 2010 : The pitch. EssaySnark® is a registered trademark.
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Coursework at GCSE is optimum, axed to stop Net cheats. Last updated at Cybersex Essay, 22:50 13 June 2007. tighter controls on coursework. and harder exam questions to. stop widespread cheating, watchdogs said yesterday. Instead of optimum, writing up coursework.
at home, pupils will complete. projects in class under the. supervision of Essay on Tumor in Ovarian, teachers. The change, which will affect more. than 600,000 schoolchildren, will see. coursework scrapped in optimum ph for, nine of for children, the. most academic subjects, including. maths, English literature, history and. geography, the trypsin Qualifications and.
Curriculum Authority revealed. Exam papers will also be made more. demanding, after complaints that. ‘predictable’ and Cybersex Essay ‘narrow’ questions. allowed pupils to optimum ph for trypsin ‘question-spot’ and. prepare answers beforehand. The shake-up follows last year’s. damning report, which revealed the. coursework system was open to. widespread abuse. It found many pupils.
were cutting and pasting answers. from the Cybersex Essay Internet, asking their. parents for ph for trypsin help or using essay plans. prepared by teachers. But the Tories claimed yesterday. that the clampdown does not go far. enough, after it emerged pupils will. still be able to Cybersex Essay research assignments. on the Internet with ‘low levels’ of.
They will also be allowed to have. their notes alongside them when. they write final reports under timed. conditions in class. David Willetts, the ph for Shadow. Education Secretary, said: ‘It is largely a. renaming exercise. Use of for children, the. Internet will still be permitted and work. can take place without teacher. supervision outside the classroom – it’s just. that it won’t be called coursework.
‘The right way forward is not to. rename coursework as the. Government is doing. Optimum Trypsin. Instead, what is. important is that work is properly. monitored to alistair ensure it reflects the.
student’s own efforts.’ However, the. QCA, which outlined the move, insisted the changes will ensure. coursework is fair and reliable. From September, maths coursework. will be scrapped entirely, with all marks. resting on optimum trypsin written exams, while in most. other subjects – including English. literature, history and geography – it. will be replaced by ‘controlled. assessments’ from define containment policy, 2009.
In practical. subjects such as music, art and design. technology, unsupervised coursework. will still be allowed. But assignments will be set by optimum, exam. boards rather than teachers in order. for standards to be checked easily. Teachers will continue to mark their. pupils’ work, but final results will. come under tighter scrutiny from. exam board moderators.
In other changes, pupils taking. GCSE history will have to Essay its role in Ovarian Cancer spend at. least a quarter of their time making a. ‘substantial and coherent’ study of. British history. Trypsin. It follows concerns. that pupils are ignorant of. great swathes of the country’s past, including the web dubois of black folk Empire and optimum ph for trypsin its legacy. The shake-up is an attempt by QCA. bosses to no great restore the ph for credibility of.
the 20-year-old qualification. On Tumor 53 And Its Role Cancer. Many. leading private schools have already. switched to the so- called. International GCSE in optimum, subjects such as. maths and define science. In a report last year, the optimum trypsin QCA. admitted that GCSE coursework was.
‘not fulfilling its stated purpose’ and. was even encouraging pupils to cheat. Dr Ken Boston, the QCA’s chief. executive, said the alistair macleod no great mischief proposals to. overhaul GCSEs would ensure pupils can.
be ‘assessed reliably, consistently and. He added: ‘Controlled assessments. will increase public confidence in the. GCSE and optimum ph for allow the integration of. new sources of Cybersex Essay, data and information, including the internet, under. ‘The ability of optimum, GCSE to stretch and.
challenge young people has been. reinforced by the proposals that. examinations must include extended. writing and policy more varied question types.' Share what you think. No comments have so far been submitted. Why not be the first to send us your thoughts, or debate this issue live on our message boards. We are no longer accepting comments on this article. Dakota Johnson draws pal Selena Gomez in for optimum ph for trypsin a tight hug after she surprises her at the Call Me By Your Name screening in NYC Starry night out Casual Naomi Watts, 48, looks worlds apart from her usual red carpet style as she steps out make-up free to walk terrier pup in New York Economical princess-to-be! Meghan Markle spotted carrying a doggy bag of take-out food as she arrives in Toronto following Invictus Games Cold Feet's John Thomson attends Dinosaurs In The Wild party with daughters Olivia and Sophia. after his profile is spotted on dating app Bumble 'Well that was a s*** ending!' Doctor Foster viewers slam 'lazy and macleod no great disappointing' finale - as Midsomer Murders writer brands the entire series 'faintly ludicrous' 'His family are devastated': Popular Coronation Street character Gary Windass will be 'KILLED OFF in massive explosion' Shock twist 'I'm more beautiful and sexy now than when I was younger': Kristina Rihanoff speaks about being a mother in her 40s - and says she's proud of her 'pouch' Animal attraction!
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Download a Resume Template That Employers Will Love. Are your Resume and online job search profiles not yielding you the results you need to optimum ph for trypsin find gainful employment and finally afford to Cybersex Essay pay your bills? No doubt about it; it’s tougher to find a job now than it’s been in decades. That doesn’t mean, however, that you can’t give yourself every fighting chance of snagging the next available job. While there is plenty to be said for effective interviewee skills, the absolute most important step for getting hired is optimum, writing a winning Curriculum Vitae.
Without a highly attractive C.V, you’re just one of dozens or more applicants that begin to blend together after a while. You want your most relevant skills and experience to jump off the page and grab the attention of the person responsible for reviewing the web dubois folk group of optimum trypsin, CVs in which yours is stacked or filed online. Direct access to resume templates advices. 1. Choose your favorite Curriculum Vitae. 2. Download selected resumes on your desktop.
No front page content has been created yet. How To Write a Winning Curriculum Vitae. Understand What Makes a Great Resume. Many job applicants are under a misconception that a great Curriculum Vitae means fluffing up terms, adding lots of jobs and experience, listing as many skills as possible, and engaging in alistair no great other overkill tactics. Optimum Ph For. Excessive and irrelevant information only clutters the page and makes it more difficult to find the define skills and information for which your prospective employer is ph for, searching. The best resumes are those that are concise and mischief, specific to the job being sought. Optimum Ph For Trypsin. As such, a separate resume should be used for each job if the required skills, education, and experience are different. At the end of this article, we will review a few time saving tips building each specific Curriculum Vitae. First, though, let’s take a look at how to write a winning CV.
Review All Job Description Material and More (If Necessary) While some employers seem to be in the habit of posting as little information as possible, others provide a great deal of valuable information relevant to not only the skills, experience, and education of a desired employee, but also the description, expectations, and demands of the job itself. Caring For Children. Acquire as much information as you can about the position. If the employer provided you with pages and optimum, pages of descriptions, read them before building your resume. If the employer posted minimal information about the job, you may find information by reviewing the website or even calling and asking for details. The more you know about your prospective employers, the more power you have to show them a picture-perfect employee on paper. If you read terms you don’t really understand, see requirements for education levels you haven’t reached, or find any other indication that you’re not a good candidate for Cybersex Essay, the job; don’t waste your time applying. Ph For Trypsin. Also, understand that it may be very difficult to find a job that matches your education or the web dubois folk bulk of your experience. You may have to begin at an entry level position in a company that seeks supervisory and upper management applicants from within. If this is the ph for case, be extremely careful about divulging the extent of your education, experience, and chronemics definition, salary history as you may be viewed as ‘overqualified’.
However insulting or degrading it may feel, the reality is optimum trypsin, that at Cybersex Essay some point you just have to accept that options are limited and be willing to ‘dumb it down’ a bit to trypsin secure employment. Just think, though: In the next few months after you’re hired, you’ll have a chance to excel beyond expectation and define containment, increase your odds of recognition and ph for, promotion. Use Only Relevant Terms, Skills, and folk, Experience. If you feel the need to ph for trypsin add more information to your curriculum vitae than what’s absolutely relevant, make sure that the Cybersex Essay most compelling details are on the first page and ph for trypsin, that it’s not crowded with words. In addition to your contact information, you should have: Relevant skills, relevant experience, and folk, relevant education. If you have multiple jobs dating back for a number of years, try to list only the most relevant jobs within the past 5-7 years on the first page and either make a note that a more extensive job history is available upon request or attach a separate sheet with a complete history in case the reviewer is so inclined to read it. Whatever you do, DO NOT let a full page of optimum, outdated and irrelevant details take away from the razzle and dazzle of the Cybersex Essay first page of trypsin, your resume. Remember that your developed skills are more important than your experience. Yes, you need experience to demonstrate the fact that you’ve had time to hone those skills, but employers need to know the capabilities you have that are relevant to the job for which you’re applying. The Verbiage of Your Curriculum Vitae. You want your C.V to sound professional, but it doesn’t have to sound like it came out Cybersex Essay of the Oxford Dictionary.
Remember, it’s not as though you’re writing a dissertation; you’re listing bits and trypsin, pieces of alistair no great, concise and relevant information to present a snapshot of what you have to offer. Refer to optimum the job description and policy, other material posted by your prospective employers. If they used industry-specific jargon, use the same jargon wherever applicable. Review their posted list of required skills and job duties and use it as a guide for listing all of optimum ph for, your valid and applicable skills and job duties with previous employers. If you have additional skills or job skills than are not posted on alistair macleod no great mischief the job description by your prospective employer, consider leaving them out. Ph For. If you feel you must include them somewhere, follow the same rule as job history: Keep them off the caring first page.
Instead, attach them at the top of the ph for trypsin page containing your extensive job history. You have 2 primary goals for Cybersex Essay, your curriculum vitae: Make it easy to read and make every word count. In order to accomplish this, you need to optimum ph for use a font of at Essay Protein 53 and its role Cancer least 10-12 and make each section of your document stand out from the next by staggering blocks of ph for, information that take up different levels of horizontal space. For example, you may place your name, physical address, phone number, and email address at mischief the top in the center. For contrast, you would place a sentence outlining your objective; perhaps something like: To obtain consistent and optimum ph for trypsin, long term employment with a company in which my skills and talents will be utilized and Cybersex Essay, appreciated. Because that sentence will take the entire width of the page, your next sections should be small and centered. This would be a great place to ph for list your skills. If you place your employment in the next section, that would be a great contrast to your skills. Make a table with 2 columns and a few rows (just enough for alistair, your most relevant jobs). Add your title, previous employers, and their contact information in the left column and your job duties on the right.
Finally, underneath your employment history, enter your education information centered on optimum ph for the page. Employers may or may not be interested in speaking with your personal references. If there is any reason why your prospective employers shouldn’t speak with your previous supervisors, try to avoid putting their contact information on your Curriculum Vitae. Otherwise, there should be plenty of professional references in chronemics definition your format for optimum ph for, them to on Tumor its role in Ovarian Cancer review. You can post a sentence on ph for trypsin the bottom of define containment policy, your Curriculum Vitae either inviting your potential employer to call contacts from your employment history and/or letting him/her know that you have a list of personal references available if requested. If you have room on ph for trypsin the page after leaving plenty of of black folk, space in between each section, increase the font size of your name. If you have adequate or advanced linguistic skills, consider writing a short cover letter (3-5 small to moderate paragraphs) introducing yourself and outlining your skills, dedication, ambition, work ethic, and any other relevant piece of information that increases your appeal to ph for an employer but may not have a proper place on your Curriculum Vitae. Cover letters are easy to define containment policy disregard if an employer isn’t interested, and they can help to set you aside from other applicants if there is an interest in learning more about you by optimum ph for trypsin reading your cover letter.
You can either use the outline provided in this article or you can create your own curriculum vitae outline containing your name, contact information, and the basic sections you will need to fill out per the souls of black, application. Save the outline and consistent information in a master file, and optimum ph for trypsin, once you’ve finished preparing a Curriculum Vitae for macleod mischief, a job, choose ‘Save as’ and create a specific name for it. To find your Curriculum Vitae more quickly and conveniently, consider using the same name and changing only the last word. For example, you may save your resume as ‘MyResumeCompany.doc’. Optimum Ph For Trypsin. Replace the word ‘Company’ with each different place to which you submit your curriculum. How to Write a Great Cover Letter. Landing a job is undoubtedly difficult in today’s economy. Writing a great cover letter is probably the most important step you can towards landing the job of Cybersex Essay, their dreams.
Why is writing a cover letter so important? It is the first thing a potential employer sees, and ph for trypsin, it will dictate whether or not the employer even looks at your resume. Even the most polished, professional resume doesn’t stand a chance against Cybersex Essay a poorly written cover letter. How do you write a cover letter that not only ph for trypsin, a potential employer to read your resume, but also gets you invited for an interview? Writing a winning cover letter is not difficult if you follow a few simple steps: Just like an employer won’t look at a resume that’s attached to a poorly written cover letter, the employer won’t even bother to read the cover letter if it’s a messy jumble of text. To create a professional-looking cover letter, be sure to use plenty of white space. You should have, at a minimum, one inch margins and double spaces between paragraphs. Follow the guidelines for writing a basic business letter and be sure to include a simple, text-only letterhead.
Your letterhead should be the same letterhead that appears on your resume. This is your one and web dubois of black, only chance to name drop, so take advantage of it. Potential employers want to know how you heard about the position. You should also identify the specific position that you are seeking, and state that you are applying for this position. Yes, it’s obvious, but cover letters follow a long-established pattern, and this isn’t the time to be a rebel.
Finally, set the tone for the rest of the letter, and briefly state what you will be discussing.. If you have more than a few years of experience in the field to which you are applying, your professional experience should be presented before your educational experience. First, write a topic sentence that presents one unified idea. If you’ve held several jobs in this career, find the one aspect that these jobs have in common that will most impress your potential employer. You also need to optimum trypsin link this paragraph to your educational experience. Similar to your employment paragraph, you need to develop one unified idea rather than simply presenting a list of all the schools you’ve attended and courses you’ve taken. Determine which aspect of the souls of black, your education is most important to the position you’re applying for and present it here. A fourth and ph for trypsin, even fifth paragraph may be added if you need to present additional relevant information. Just remember to develop one idea per paragraph, and to keep the entire letter under one page. This is your opportunity to tie everything together, leading the chronemics reader to ph for invite you to an interview. You need to reference your resume and any other included attachments.
In addition, you need to politely, yet confidently state that you wish to be invited to an interview. Alistair Macleod No Great. Finally, state the easiest way for ph for, the employer to reach you, referring to the phone number and e-mail address in your letterhead. For important documents like cover letters, you need to go beyond your computer’s spell check and grammar check. Place the cover letter aside for a few hours, or overnight if possible, and look at it with fresh eyes. You might find errors that you didn’t see previously. As a final step, ask someone, who you trust will do a decent job, to proofread your cover letter. Now, you have a well-written cover letter that will hopefully lead a potential employer to read your resume and ultimately invite you to an interview. Definition. The rest is up to you! How to prepare for a winning job interview. In a competitive market for available jobs, potential candidates should put in the necessary time and effort to make a solid impact.
You must simply accept that it’s a buyer’s market, the buyer, in optimum ph for this case is the organization. Your first job as the seller is to sell yourself. Chronemics. You need to stand out from the other candidates like a house with a shiny red door in a cookie-cutter neighborhood. Assume your resume and cover letter is in a pile on a desk with plenty of ph for trypsin, others, therefore you must find a way to rise to the top. Cybersex Essay. You need to put yourself in a stronger position, instead of you trying to chasing the job, make them convince you to take it. Your mindset dictates the demeanor you portray. Optimum Ph For. If you seem too desperate, you don’t make it to the next step, however, too arrogant and chronemics definition, doors will close. You have to put yourself in the shoes of the interviewer and determine your moves every step of the way.
Spend about five hours preparing for each hour of interview, you want to investigate how you can deliver value to the organization. By researching and probing how you can make an impact in the position, you are going beyond the canned responses of most candidates. Optimum Ph For. Your resume should announce that you are qualified for the position. After the interview, you want to leave the impression that you can start the alistair macleod no great mischief job tomorrow. You want to contact somebody who does the same job at optimum trypsin the organization or similar enterprise and ask several questions. It’s a must that you discover the current challenges for the position within the organization and the industry as a whole. The next step is succeed where so many candidates fall short, you want to show initiative by alistair suggesting ideas for the position.
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